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EASYspin Plus 组织/细胞RNA快速提取试剂盒

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EASYspin Plus 组织/细胞RNA快速提取试剂盒详细说明

产品介绍:

    本公司独推出EASYspin无苯酚、氯仿RNA快速提取技术基础上,又独研发成功基因组DNA去除柱技术确保有效去除gDNA残留,得到的RNA不需要DNase消化,可直接用于PCR、荧光定量PCR等实验。独特的裂解液迅速裂解细胞和灭活细胞RNA酶,然后裂解混合物通过一个基因组DNA去除柱,基因组DNA被去除而RNA穿透滤过。滤过的RNA用乙醇调节结合条件后,RNA在高离序盐状态下选择性吸附于离心柱内硅基质膜, 再通过一系列快速的漂洗-离心的步骤, 去蛋白液和漂洗液将细胞代谢物,蛋白等杂质去除, 而后低盐的RNase free H20将纯净RNA从硅基质膜上洗脱。


产品特点:1.完全不使用有毒的苯酚,氯仿,beta巯基乙醇等试剂,也不需要乙醇沉淀等步骤。

2.快速,简捷,单个样品操作一般可在30分钟内完成。

3.成功研发基因组DNA去除柱技术确保有效去除gDNA残留,得到的RNA不需要DNase消化,可直接用于PCR、荧光定量PCR等实验。

4.多次柱漂洗确保高纯度,OD260/OD280典型的比值达1.9~2.0,基本无DNA残留,可用于RT-PCR,Northern-blot和各种实验。


RN28 EASYspin plus 组织细胞RNA发表文章:

1. Engagement of Soluble Resistance-Related Calcium Binding Protein (sorcin) with foot-and-mouth disease virus (FMDV) VP1 inhibits type I interferon response in cells. Available online 21 May 2013

2. Overexpression of small heat shock protein 21 protects the Chinese oak 4 silkworm Antheraea pernyi against thermal stress. Journal of Insect Physiology, IP 3099 No. of Pages 7, Model 5G 14 June 2013

3. S-Adenosylmethionine-induced adipogenesis is accompanied by suppression of Wnt/b-catenin and Hedgehog signaling pathways. Mol Cell Biochem (2013)382:59–73,DOI 10.1007/s11010-013-1718-3

4. A critical role of interferon-induced protein IFP35 in the type I interferon response in cells induced by foot-and-mouth disease virus (FMDV) protein 2C. Archives of Virology 2014, DOI 10.1007/s00705-014-2147-7

5. Saikosaponin-D Attenuates Heat Stress-Induced Oxidative Damage in LLC-PK1 Cells by Increasing the Expression of Anti-Oxidant Enzymes and HSP72. The American Journal of Chinese Medicine, 2014, 42(5): 1–17

6. The Cellular Prion Protein Negatively Regulates Phagocytosis and Cytokine Expression in Murine Bone Marrow-Derived Macrophages. PLoS ONE 2014, 9(7): e102785. doi:10.1371/journal.pone.0102785

7. Critical role of eukaryotic elongation factor 1 alpha 1 (EEF1A1) in avian reovirus sigma-C-induced apoptosis and inhibition of viral growth. Archives of Virology 2015, 160:1449–1461

8. Baicalin protects sertoli cells from heat stress-induced apoptosis viaactivation of the Fas/FasL pathway and Hsp72 expression. Reproductive Toxicology 2015, Accepted 12 June 2015

9. Identification and expression of an ecdysteroid-responsive amylase from red crayfish Procambarus clarkii. Fisheries Science 2015, 81(2): 345-352

10. Small Heat Shock Proteins and Eicosanoid Pathways Modulate Caspase-1 Activity in the Fat Bodies of Antheraea pernyi. first posted online September 3, 2015; doi: 

11. Inflammes-dependent regulation of IL-1b secretion induced by the virulent Mycobacterium bovis Beijing strain in THP-1 macrophages.Antonie van Leeuwenhoek 2015, DOI 10.1007/s10482-015-0475-6

12. Baicalin protects sertoli cells from heat stress-induced apoptosis via activation of the Fas/FasL pathway and Hsp72 expression. Reproductive Toxicology 2015, 57: 196-203

13. Plumbagin Ameliorates CCl4-Induced Hepatic Fibrosis in Rats via the Epidermal Growth Factor Receptor Signaling Pathway. Evidence-Based Complementary and Alternative Medicine 2015

14. Protection of Bovine Mammary Epithelial Cells from Hydrogen Peroxide-Induced Oxidative Cell Damage by Resveratrol. Oxidative Medicine and Cellular Longevity 2016, Article ID 2572175, 15 pages

15. Metabonomics applied in exploring the antitumour mechanism of physapubenolide on hepatocellular carcinoma cells by targeting glycolysis through the Akt-p53 pathway. Scientific Reports 2016, 6:29926 | DOI: 10.1038/srep29926

16. Hesperidin prevents lipopolysaccharide-induced endotoxicity in rats.I mmunopharmacology And Immunotoxicology 2016, 38(5): 364–371

17. A study of the damage of the intestinal mucosa barrier structure and function of Ctenopharyngodon idella with Aeromonas hydrophila. Fish Physiology and Biochemistry 2017, 43(5): 1223–1235

18. Both point mutations and low expression levels of the nicotinic acetylcholine receptor β1 subunit are associated with imidacloprid resistance in an Aphis gossypii (Glover) population from a Bt cotton field in China. Pesticide Biochemistry and Physiology 2017, 141: 1-8

19. Silencing of histone methyltransferase NSD3 reduces cell viability in osteosarcoma with induction of apoptosis. Oncology Reports 2017, 2796-2802

20. Acute aflatoxin B1 – Induced hepatotoxicity alters gene expression and disrupts lipid and lipoprotein metabolism in rats. Toxicology Reports 2017, 4: 408-414(出口产品发的文章)

21. Cloning and expression of three thaumatin-like protein genes from Polyporus umbellatus. Acta Pharmaceutica Sinica B 2017, 3: 373-380

22. Structural analysis and immunomodulatory effect of polysaccharide from Atractylodis macrocephalae Koidz. on bovine lymphocytes. Carbohydrate Polymers 2017, 174: 1213-1223

23. Transcriptome profiling of spleen provides insights into the antiviral mechanism in Schizothorax prenanti after poly (I: C) challenge. Fish & Shellfish Immunology 2017, 62: 13-23

24. Front. Microbiol., 19 July 2017

25. VP2 of Infectious Bursal Disease Virus Induces Apoptosis via Triggering Oral Cancer Overexpressed 1 (ORAOV1) Protein Degradation. Front. Microbiol. 2017

26. MicroRNA-20a contributes to cisplatin-resistance and migration of OVCAR3 ovarian cancer cell line. Oncology letters. 2017, 1780-1786

27. Down-regulation of aquaporin 5-mediated epithelial-mesenchymal transition and anti-metastatic effect by natural product Cairicoside E in colorectal cancer. Molecular Carcinogenesis 2017, 11 September 2017 DOI: 10.1002/mc.22712  View/save citation

28. The impact of Zearalenone on the meiotic progression and primordial follicle assembly during early oogenesis. Toxicology and Applied Pharmacology 2017, 329: 9-17

29. Benzo[a]pyrene exposure under future ocean acidification scenarios weakens the immune responses of blood clam, Tegillarca granosa. Fish & Shellfish Immunology 2017, 63: 465-470

30. Transcriptome analysis of sex-related genes in the blood clam Tegillarca granosa. PLoS ONE 12(9): e0184584

31. Oral Administration of Lactobacillus rhamnosus GG Ameliorates Salmonella Infantis-Induced Inflammation in a Pig Model via Activation of the IL-22BP/IL-22/STAT3 Pathway. Front. Cell. Infect. Microbiol. 2017, |



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